Figure 2
From: RNA activation in ticks
Normal expression of HlemCHT in H. longicornis. (a) RT-PCR analysis of HlemCHT in different developmental stages of H. longicornis. Analysis was performed using total RNA from eggs (12 and 15 Dpo), the whole body of larvae (unfed, partially fed, engorged), nymph (unfed, partially fed, engorged), and adult (unfed, partially fed, engorged). H. longicornis 40S ribosomal protein S3a was used as an internal control. PCR products were analysed on 1.5% agarose gel and stained with ethidium bromide for viewing. Original gels are presented in Supplementary Fig. S1. (b) RT-qPCR analysis of HlemCHT during embryogenesis of tick eggs. The expression of each mRNA was normalized to the expression of 40S ribosomal protein S3a measured in same RNA preparation. The relative expression values are represented as “means ± SD”. Error bars represent the SEM of 4 replicates. *Indicates a difference at P < 0.05; **designates P < 0.005; ***designates P < 0.001. The results shown are from a single experiment and are representative of three independent experiments. n.d. not determined.
