Figure 4

ebony mutants have fewer but longer sleep bouts and similar activity levels compared to controls. (A–B) Schematics of: (A) the setup of a Drosophila Activity Monitor (DAM); and (B) typical bouts of activity (wake) and inactivity (sleep) over a 1-hour period. (C) Plots show the average activity (top) and sleep duration per 30 minutes (bottom) over a 24-hour period for control flies (gray) and ebony mutants (green). (D–J) Relative to controls (gray), ebony mutants (green) exhibited: (D) similar average activity per hour (p = 0.4372); (E) increased sleep amount over a 24-hour period (p < 0.0001); (F) decreased average daytime bout number (p = 0.0038); (G) decreased average nighttime bout number (p = 0.0001); (H) increased average bout length (p=0.0002); (I) increased average daytime bout length (p = 0.0006); and (J) increased average nighttime bout length (p=0.0005). Each data point represents a single fly (n = number of flies); p-values were obtained by unpaired Student’s t-test with Welch’s correction (D–G) and Mann-Whitney U test (H–J). Averages are shown with error bars representing SEM. (See Table S1 for n and statistical analysis for each experiment; n.s.= p > 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.)