Figure 6

Liraglutide regulated L6 myotube IR by activating SESN2. (A) The glucose oxidase–peroxidase micromethod was used to determine the level of glucose in the cell supernatant. (B) The mRNA levels of GLUT4 in L6 myotubes were determined using quantitative reverse transcriptase-polymerase chain reaction. (C) and (D) The protein levels of GLUT4 and p-Akt/Akt in L6 myotubes were determined using western blotting. The results are expressed as means ± S.D. of three independent experiments. *P < 0.05 vs siCON.