Figure 2 | Scientific Reports

Figure 2

From: Development of a novel air–liquid interface airway tissue equivalent model for in vitro respiratory modeling studies

Figure 2

Fabrication & analysis of lung extracellular matrix-derived biogel. (a) To obtain the lung ECM biogel, human lung tissue is decellularized, lyophilized and cryomilled, digested, and mixed with a HA and gelatin-based hydrogel to form the biogel. (b) Utilizing ECM colorimetric kits and ELISAs, specific ECM components were quantified for each lung sample (n = 6). The ECM biogel can employ crosslinking molecules of different sizes and geometries to tune the biomechanical properties of the hydrogel, including elastic modulus (c) and pore size (d), measured via SEM imaging (e).

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