Figure 3

Epithelial barrier functionality analysis. (a) Representative whole mount images of the epithelial surface of the OTE cultures during the culture period. (b) Epithelial confluency was quantified and plotted for the duration of the culture (n = 6). (c) Trans-epithelial electrical resistance (TEER) of 3D OTE HBE cells during the entire culture period (n = 12). Significance compared the final day of TEER measurements and the vertical dotted line signifies the day the cultures were changed to ALI. (Note: Some error bars are too small to be shown).