Figure 4

Epithelial pseudostratification analysis. (a) Representative H&E staining of the FB + /ECM + OTE model groups, 2D ALI Culture, and human airway tissue at ALI day 28. (Scale bar = 50 μm). (b) Quantitative analysis of the epithelial thickness at ALI day 28 completed via MATLAB image segmentation (n = 6). The significance is in relationship to human airway epithelium (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).