Figure 5 | Scientific Reports

Figure 5

From: Development of a novel air–liquid interface airway tissue equivalent model for in vitro respiratory modeling studies

Figure 5The alternative text for this image may have been generated using AI.

Multispectral analysis of epithelial differentiation markers. (a) Representative multispectral staining of human airway tissue, 2D ALI culture, and the complete OTE models for detection of the following markers: DAPI (blue), acetylated tubulin (green), MUC5B (white), MUC5AC (red), and TP63 (yellow) (Scale bar = 25 μm). (b) Associated quantitative analysis of HBE differentiation markers (acetylated tubulin, MUC5AC, MUC5B, and TP63) between the standard 2D ALI culture, OTE models, and human airway tissue (n = 6). (ns not significant, **p < 0.01, ***p < 0.001, ****p < 0.0001).

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