Figure 1
Treg depletion induced long-term tumor rejection responses. (A) Schematic illustration of the rechallenged CMS5 tumor rejection model. DEREG mice were inoculated s.c. with CMS5 tumor cells. DT was injected i.p. to delete Tregs. After 25 days or 3 months, DEREG mice were rechallenged with CMS5. (B) Tumor development after CMS5 primary challenge (n ≥ 8). (C) Tumor development after CMS5 rechallenge (n ≥ 8). (D) Schematic illustration of the rechallenged 4T-1 tumor rejection model. The 4 T-1 tumor was challenged in this protocol. In the rechallenge process, tumors were inoculated s.c. on the right flank (Re.4T-1 ipsilateral) or the left flank (Re.4T-1 contralateral). (E) Tumor growth after 4T-1 challenge (n = 8). (F) Tumor growth after 4T-1 rechallenge (n = 8). Two-way ANOVA followed by multiple comparisons for tumor growth curve analysis was used. Error bars represent mean \(\pm\) SEM.
