Figure 3

The rechallenged tumors were rejected even with circulating immune cell depletion. (A) Schematic illustration of immune cells depletion with antibodies during tumor rechallenge. (B) Tumor development with CD4⁺ T cells depletion (n = 5). (C) Tumor development with CD8⁺ T cells depletion (n = 5). (D) Tumor development with the depletion of CD4⁺ T and CD8⁺ T cells (n = 5). (E) Tumor growth with NK cell depletion (n = 5). (F) Tumor growth with neutrophil depletion (n = 5). (G) Tumor development with FTY720 treatment (n = 5). Two-way ANOVA followed by multiple comparisons for tumor growth curve analysis was used. Error bars represent mean \(\pm\) SEM. See also Fig. S3.