Figure 2 | Scientific Reports

Figure 2

From: Modelling ligand depletion for simultaneous affinity and binding site quantification on cells and tissue

Figure 2

Representative binding traces for radioligands. Peptide tracer binding to human GIPR-expressing CHO cells (a,b), peptide tracer binding to FAP-expressing HEK cells (c,d), low molecular weight tracer binding to PSMA-expressing PC3-PIP cells (f,g) and anti-PSMA antibody binding to PSMA-expressing PC3-PIP cells (i,j). Binding traces are displayed in black and fitted lines in red: fitted with the 1:1 model (a,c,f,i) and with the 1:1 depletion corrected model (b,d,g,j), respectively. Cell number used per replicate plotted against number of binding sites (nB) estimated by the depletion model for the corresponding model system (e,h).

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