Figure 3
Differentially expressed genes in the general microvascular endothelial cell cluster (mvEC) predicted increased inflammation and production of reactive oxygen species in CDH lungs. Differential gene expression was determined for both CDH and NC lungs compared to healthy controls and analyzed by Ingenuity Pathway Analysis (IPA). Expression of genes attached to biological pathways detected by IPA are shown in heatmaps as differentially expressed in both CDH and NC lungs (a), differentially expressed in only CDH lungs (b) and differentially expressed in only NC lungs (c). Differentially expressed mvEC genes identified in the heatmaps are sorted by smallest to largest log2FC. Several biological processes related to inflammation and free radical production were predicted by IPA to be upregulated in E21.5 CDH mvECs (d). Increased inflammation was not predicted by IPA for the NC mvECs (e). Rather, the NC mvECs were enriched for necrosis, apoptosis and notable for reduced cell viability. Activation states for the different biological processes were inferred from z-scores, which relate experimentally observed differential gene expression with literature-derived directions of effect to predict implicated biological functions independent of the associated p-values. Activation z-scores ≥|2| were considered most significant by convention.
