Figure 3 | Scientific Reports

Figure 3

From: Dynamic in vitro culture of bovine and human ovarian tissue enhances follicle progression and health

Figure 3

Histological characterization of fresh and cultured BOCT. (a) Follicle quality in BOCT cultured under static (CD and PD) and dynamic (PB) conditions versus fresh tissue (D0). (b-d) Representative images of grade I (b), II (c) and III (d) follicles. (e) Follicle stages in BOCT cultured in static (CD and PD) and dynamic (PB) conditions versus fresh tissue (D0); (f–h) Representative images of primordial (f), primary (g) and secondary (h) follicles. Bar = 20 μm. All the experiments were performed in triplicate (n = 3), and the values represent cumulative percentages. *P < 0.05 and **P < 0.01 versus D0; #P < 0.05 and ##P < 0.01 PB versus PD; §§P < 0.01 PB versus CD; ^P < 0.05 and ^^P < 0.01 PD versus CD.

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