Figure 3
From: Dynamic in vitro culture of bovine and human ovarian tissue enhances follicle progression and health

Histological characterization of fresh and cultured BOCT. (a) Follicle quality in BOCT cultured under static (CD and PD) and dynamic (PB) conditions versus fresh tissue (D0). (b-d) Representative images of grade I (b), II (c) and III (d) follicles. (e) Follicle stages in BOCT cultured in static (CD and PD) and dynamic (PB) conditions versus fresh tissue (D0); (f–h) Representative images of primordial (f), primary (g) and secondary (h) follicles. Bar = 20 μm. All the experiments were performed in triplicate (n = 3), and the values represent cumulative percentages. *P < 0.05 and **P < 0.01 versus D0; #P < 0.05 and ##P < 0.01 PB versus PD; §§P < 0.01 PB versus CD; ^P < 0.05 and ^^P < 0.01 PD versus CD.