Figure 4
From: Dynamic in vitro culture of bovine and human ovarian tissue enhances follicle progression and health
Follicle viability in fresh and cultured BOCT. (a) Follicle viability of BOCT cultured under static (CD and PD) and dynamic conditions (PB) versus fresh tissue (D0). (b, c) Representative confocal micrographs of a live (b), and a dead (c) follicle. Blue, Hoechst 33342 ± stained nuclei; red, extracellular matrix and dead cells stained by live ± dead far-red; magenta, nuclei of dead cells. Bar = 20 μm. All the experiments were performed in triplicate (n = 3), and the values represent cumulative percentages. **P < 0.01 versus D0; §§P < 0.01 PB versus CD; ^^P < 0.01 PD versus CD.
