Table 2 Total sequencing output by tick sample generated during NAS and control sequencing experiments.

From: Metagenomic surveillance for bacterial tick-borne pathogens using nanopore adaptive sampling

 

Total reads

Total Bases sequenced (Mb)

Read length N50 (bp)

Mean read quality

NAS enrichment

   Tick 1

1,154,447

421.4

397

9.9

   Tick 2

1,115,550

390.2

376

9.9

   Tick 3

644,767

233.8

400

10

   Tick 4

1,853,677

662.8

386

9.9

   Total

4,768,441

1708.2

389

9.8

Control

   Tick 1

579,959

331.7

771

10

   Tick 2

606,365

302.3

605

10.1

   Tick 3

360,105

226.8

1,005

10.1

   Tick 4

959,027

654.8

1,185

10.1

   Total

2,505,456

1515.7

888

10.1

NAS depletion

   Tick 5

2,233,967

621.3

328

13.7

   Tick 6

1,494,667

392.9

317

13.6

   Tick 7

712,929

186.5

312

13.6

   Tick 8

837,779

247.5

348

13.5

   Total

5,279,342

1448.1

327

13.5

  1. Total sequencing output and summary statistics for Ixodes scapularis tick samples used for both NAS enrichment, NAS depletion, and control MinION experiments.