Table 3 Taxonomic classification results for reads generated during NAS and control experiments.

From: Metagenomic surveillance for bacterial tick-borne pathogens using nanopore adaptive sampling

 

Bacterial tick-borne pathogen genome

A. phagocytophilum

B. burgdorferi s.s

B. miyamotoi

E. muris eauclairensis

A. phagocytophilum

B. burgdorferi s.s

B. miyamotoi

E. muris eauclairensis

 

NAS enrichment

Control

Tick 1

0

274(2.4)

0

769(2.1)

1

113

0

364

Tick 2

0

351(1.8)

5

0

0

194

4

1

Tick 3

0

334(1.8)

1113(2.1)

2

1

190

523

2

Tick 4

238(1.8)

218(1.7)

5

2

136

127

2

1

 

NAS depletion

 

Tick 5

153

657

0

0

    

Tick 6

665

1457

1

146

    

Tick 7

4

190

0

0

    

Tick 8

14

422

1

0

    
  1. Numbers presented under each TBP taxonomic group indicate the number of ONT reads successfully identified taxonomy at the species level using the kraken2 metagenomic classification pipeline. Numbers within parentheses denote the fold enrichment achieved in classified reads between ticks sequenced during paired adaptive sampling and control experiments.