Table 1 List of primers used for quantitative real-time PCR.
Gene symbol | Primers | |
|---|---|---|
Human | ||
GAPDH | SYBR | F: 3′-CAATGACCCCTTCATTGACC-5′ R: 5′-TTGATTTTGGAGGGATCTCG-3′ |
LIN28 | SYBR | F: 3′-CACGGTGCGGGCATCTG-5′ R: 5′-CCTTCCATGTGCAGCTTACTC-3′ |
NANOG | SYBR | F: 3′-CTCAGCTACAAACAGGTGAAGAC-5′ R: 5′-TCCCTGGTGGTAGGAAGAGTAAA-3′ |
OCT4 | SYBR | F: 3′-GAAACCCACACTGCAGCAGA-5′ R: 5′-TCGCTTGCCCTTCTGGCG-3′ |
SOX2 | SYBR | F: 3′-GCGCCCTGCAGTACAACTC-5′ R: 5′-CGGACTTGACCACCGAACC-3′ |
TNNT2 | SYBR | F: 3′-GGCAGCTCCTGTTTGGAAATG-5′ R: 5′-TTATTACTGGTGTGGAGTGGGTGTG-3′ |
ACTN2 | SYBR | F: 3′-TTTCCCTGTGTGTTGGTTGC-5′ R: 5′-TGATTACACTCCGCACATTTCA-3′ |
