Table 1 Experimental design and sample collection.

Pharmacokinetic profiles

Rats

³H-L-S-CPP

3

6.3 × 10⁷ dpm  kg⁻¹ (D_o)

(32.0 ug  kg⁻¹)

0.016, 0.033, 0.083, 0.166, 0.5, 1, 2, 5, and 24 h after administration

³H-D-S-CPP

3

16.2 × 10⁷ dpm  kg⁻¹ (D_o)

(3.5 ug  kg⁻¹)

³H-IgG

3

12.8 × 10⁶ dpm  kg⁻¹ (D_o)

(19.1 ug  kg⁻¹)

 ± L-S-CPP 1.4 mg  kg⁻¹

(250 µM)

0.016, 0.033, 0.083, 0.166, 0.5, 1, 2, 5, 24 and 48 h after administration

³H-IgG + L-S-CPP

3

Biodistribution

Mice

³H-L-S-CPP

2–4 per group

10.4–15.9 ug  kg⁻¹

1 and 5 h after administration

³H-D-S-CPP

³H-IgG_antiNUP

6

82.5 ug  kg⁻¹

 ± L/D-S-CPP or Scramble ≈ 3.6 mg  kg⁻¹

1 h after administration

³H-IgG_antiNUP + L-S-CPP

11–15 per group

³H-IgG_antiNUP + D-S-CPP

³H-IgG_antiNUP + Scramble

Toxicology

Mice

L-S-CPP

4–5 per group

3.6 mg  kg⁻¹

120 h

D-S-CPP

PBS

BBB Transport

Mice

³H-L-S-CPP

15

0.2–0.8 µCi  ml⁻¹

Corresponding to 0.2–0.8 µg (≈7.0-27 µg  kg⁻¹)

60 s