Figure 3

Olfm4/eGFP is expressed during early postnatal development of Olfm4^eGFP mouse prostate and urethral epithelium. (a, b) Representative merged and single-color images from triple-color immunofluorescent staining in P1 (a) or P7 (b) prostate tissues of Olfm4^eGFP mice with Olfm4, E-cadherin, and GFP (ab13970, Abcam) antibodies (left panels) or with Olfm4, Foxa1, and GFP (ab13970, Abcam) antibodies (right panels). Ecd, E-cadherin. Scale bars: 20 μm. Blue represents DAPI nuclear staining. # indicates the region shown in the single-color staining micrographs. (c, d) Representative merged images from triple-color immunofluorescent staining in P14 (c) or P20 (d) urethral-tube or prostate tissues of Olfm4^eGFP mice with Olfm4, Foxa1, and GFP (ab13970, Abcam) antibodies. UT, urethral tubes; AP, anterior prostate; DLP, dorsolateral prostate; VP, ventral prostate. Scale bars: 20 μm. Blue represents DAPI nuclear staining.