Figure 4

Olfm4+ cells persist during late postnatal development of Olfm4eGFP mouse anterior prostate and urethral epithelium. (a, b) Representative merged images from triple-color immunofluorescent staining in P42 urethral-tube (a) and prostate (b) tissues of Olfm4eGFP mice with Olfm4, Foxa1, and Sca-1 or Ck5 antibodies. UT, urethral tubes; AP, anterior prostate; DLP, dorsolateral prostate; VP, ventral prostate. Scale bars: 20 μm. Blue represents DAPI nuclear staining. (c, d) Representative single-color or merged images from triple-color immunofluorescent staining in P42 (c) and P56 (d) urethral tubes of Olfm4eGFP mice with Olfm4, Ck5, and Ck4 or Ck8 antibodies. Scale bars: 20 μm. Blue represents DAPI nuclear staining.