Table 4 The inhibitory effect of isolated LAB strains against pathogens.

From: Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Pathogens

Origin

Incubation conditions

Diameter of inhibition zone (mm)

C3

C18

C20

C39

C58

CON

S. sanguinis

PTCC 1449

37 °C in TSA II medium

13.6 ± 0.3c

19.0 ± 0.2a

16.1 ± 0.2b

12.3 ± 0.6d

18.9 ± 0.5a

0.0 ± 0.0e

S. salivarius

PTCC 1448

37 °C in TSA II medium

16.3 ± 0.4c

20.9 ± 0.5a

16.7 ± 0.2c

18.8 ± 0.5b

21.3 ± 0.8a

0.0 ± 0.0d

S. sobrinus

PTCC 1601

37 °C in Blood agar medium

18.3 ± 0.7b

21.6 ± 0.4a

17.2 ± 0.5c

17.4 ± 0.4bc

21.2 ± 0.9a

0.0 ± 0.0d

Y. enterocolitica

ATCC 23,715

37 °C in TSA medium

15.8 ± 0.4d

20.9 ± 0.4a

16.4 ± 1.1d

17.8 ± 0.8c

19.3 ± 0.9b

0.0 ± 0.0e

S. mutans

PTCC 1683

37 °C in MHA medium

15.7 ± 0.4b

25.4 ± 0.6a

14.2 ± 0.4c

13.3 ± 0.2d

14.4 ± 0.3c

0.0 ± 0.0e

P.aeruginosa

PTCC 1181

26 °C in Nutrient agar medium

14.3 ± 0.4c

21.5 ± 0.4a

15.7 ± 0.2b

14.3 ± 0.3c

14.6 ± 0.4c

0.0 ± 0.0d

S. aureus

ATCC 25,923

37 °C in Blood agar medium

15.7 ± 0.3c

21.8 ± 0.4a

16.4 ± 0.6b

15.3 ± 0.4c

21.7 ± 0.2a

0.0 ± 0.0d

B. subtilis

ATCC 19,652

30 °C in Nutrient agar/broth medium

15.1 ± 0.3c

21.6 ± 0.3a

12.3 ± 0.6d

16.3 ± 0.4b

21.5 ± 0.1a

0.0 ± 0.0e

L. monocytogenes

ATCC 13,932

37 °C in BHI medium

16.2 ± 0.4d

18.8 ± 0.6c

15.7 ± 0.2d

20.0 ± 0.5b

20.9 ± 0.5a

0.0 ± 0.0e

K. pneumoniae

PTCC 1053

37 °C in MPA medium

20.2 ± 0.4b

18.7 ± 0.2d

16.1 ± 0.2e

19.7 ± 0.3c

21.0 ± 0.4a

0.0 ± 0.0f

S. flexneri

PTCC 1234

37 °C in MHA medium

18.6 ± 0.3b

23.8 ± 0.7a

14.1 ± 0.2d

15.2 ± 0.4c

19.0 ± 0.4b

0.0 ± 0.0e

E. coli

PTCC 1276

37 °C in LB medium

15.8 ± 0.5c

20.9 ± 0.4a

16.8 ± 0.5b

10.2 ± 0.4d

20.8 ± 0.3a

0.0 ± 0.0e

  1. Values are mean ± standard error of triplicates. a-f Means in the same row with different lowercase letters differed significantly (P < 0.05).
  2. ATCC: American Type Culture Collection, Virginia, USA. PTCC: Persian Type Culture Collection, Tehran, Iran.
  3. Streptococcus sanguinis, Streptococcus salivarius, Streptococcus sobrinus, Yersinia enterocolitica, Streptococcus mutans, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Listeria monocytogenes, Klebsiella pneumoniae, Shigella flexneri, and Escherichia coli. These pathogenic organisms were purchased from the Persian Type Culture Collection (PTCC).