Figure 2
From: Long-term in vivo vitamin D3 supplementation modulates bovine IL-1 and chemokine responses
Vitamin D supplemented calves (VD) exhibit 27 differentially expressed genes in comparison to controls in unstimulated whole blood (Con). (a) PCA analysis of unstimulated gene expression of 77 genes in Con and VD calves. (b) Heatmap of relative unstimulated expression (mean centred dCt) of 77 genes in Con and VD calves and p value results of multiple t tests after p value adjustment: *p < 0.1. (c) Radar plot of the relative expression (mean centred dCt) of the 27 genes found differentially expressed between Con and VD calves. (d) Unstimulated gene expression z-scores of gene groups in Con and VD calves; *p < 0.05, **p < 0.01, ***p < 0.001. (e) Protein expression of IL-1β, IL-6 and IL-8 in unstimulated samples supernatant at 3, 5 and 7 months of age in Con and VD calves, with lower limit of detection (LLD) of ELISA labelled for IL-1β and IL-6; *p < 0.05, **p < 0.01.
