Figure 2
Expression of BMPR1A and BMPR2 was increased in inflamed and recovering mesothelial cells (IC, WB, and statistical analyses). (A) Dot-like BMPR1A expression could be detected in control mesothelial cells (CTR). (B) On the 3rd day after Freund’s adjuvant injection (D3), BMPR1A expression was weakly decreased. (C) On the 5th day (D5), the presence of BMPR1A was more pronounced in the mesentery, while just a moderately increased in mesothelial cells. (D) On day 8 (D8), the expression of BMPR1A was slightly stronger. (E) At the end of the regeneration (D11), mesothelial cells were still BMPR1A positive. (F) The WB analysis showed that BMPR1A was already expressed in control mesothelial cells (CTR), its expression level was high on day 5 and 11 (D5, D11). Statistical analysis of WB densitometry data on BMPR1A expression in mesothelial cell lysates (*p < 0.05). β-actin was used as a loading control. (G) BMPR2 expression was barely detectable in control cells (CTR). (H) On day 3 (D3), strong, specific, punctate-like BMPR2 signals could be observed in the cytoplasm of mesothelial cells. (I) On the 5th day (D5), the receptor expression was enhanced. (J,K) As the regeneration started (D8, D11), BMPR2 expression was still intense. (L) WB results and the densitometry showed that the BMPR2 expression level was gradually increased over time. Statistical analysis of WB densitometry data on BMPR2 expression in mesothelial cell lysates (*p < 0.05). β-actin was used as a loading control. (M) Statistical comparison of BMPR1A and BMPR2 expression in mesothelial cells (***p < 0.001). (Arrowheads: mesothelial cells; LYS: mesothelial cell lysate; blue: DAPI; green: (A–E) BMPR1A, (G–K) BMPR2; WB images were cropped; original blots are presented in Supplementary Fig. S2; data of the statistical analyses and cell numbers of the groups are presented in Supplementary Tables S1, S2). Bar ~ 10.80 µm.
