Figure 4

Agarose gel electrophoresis visualization of kDNA, ITS1 and ITS1-RFLP. (A1) Minicircle kDNA PCR-1 assay of clinical samples. (A2) Minicircle kDNA PCR-2 assay of clinical samples. PCR 1 amplicons of DNA isolated from the skin biopsies run for gel electrophoresis on 1.5% agarose gel and analysis. B1) ITS1 PCR assay of clinical samples. DNA isolated from the skin biopsies from the lesion was amplified and analyzed on 1.5% agarose gel. B2) ITS1 RFLP assay of ITS1 PCR positive samples. The grouping of the gels cropped were from different gels. The full length gels are included in supplementary information file. The size of the bands are defined as indicated by the arrows. Ladders-100 bp DNA ladder (Solis Biodyne), NC-Negative Control, L.D.- Control of L. donovani (LEM 138), CL- Cutaneous leishmaniasis sample.