Figure 3
Effect of the stage of the cycle (a) and anatomical regions (b) from which the oviduct fluid originated on sperm binding to oviduct epithelial spheroids (Experiment 2). The spermatozoa and spheroids were incubated for 60 min in the TLP medium (control) or in the OF collected from whole oviducts at the pre-ovulatory (Pre-ov) or luteal (Luteal) phases of the cycle (a), or from the isthmus (Isth) or ampulla (Amp) at pre-ovulatory (Pre-ov) or post-ovulatory (Post-ov) phases of the cycle. All of the OF samples used had a final protein concentration of 1 mg/mL. The data are provided as the mean ± SEM of bound sperm per mm2 relative to the control (n = 3 replicates).
