Figure 4
Effect of ultra-filtrated fractions of pre-ovulatory oviduct fluid on sperm binding to oviduct epithelial spheroids (Experiment 3). The spermatozoa and spheroids were incubated for 60 min with pre-ovulatory OF after ultrafiltration with a cut-off of 3 kDa. Native OF at a final protein concentration of 1 mg/mL was included (OF). The TLP medium was used as the control. The data are presented as the mean ± SEM of bound sperm per mm2 relative to the control (n = 4 replicates).
