Figure 6
Effect of hyaluronic acid and heparan sulphate on sperm density on bovine oviduct epithelial spheroids (Experiment 5). The spermatozoa and spheroids were incubated for 60 min with hyaluronic acid (HA) at 10, 100, and 1000 µg/mL (a), or with heparan sulphate (HS) at 10, 100, and 1000 µg/mL and OF in which the HS was digested with heparinases (OF-HS, b). Native OF at a final protein concentration of 1 mg/mL was included (OF) and the TLP medium was used as control in each experiment. The data are presented as the mean ± SEM of bound sperm per mm2 relative to the control (n = 4 replicates for each experiment).
