Figure 5 | Scientific Reports

Figure 5

From: Acute muscle mass loss was alleviated with HMGB1 neutralizing antibody treatment in severe burned rats

Figure 5

Histological evaluation of inflammatory infiltrate in hind limb muscles of burn rats (± HMGB1 Ab). Sprague Dawley rats were subjected to burn, and immediately treated with vehicle or anti-HMGB1 antibody (controls: sham burn). Paraffin-embedded hind limb tissue Sects. (5 μM) were examined by hematoxylin/eosin staining (blue: nuclear; pink: muscle/cytoplasm). Shown are representative H&E-stained images of plantaris (a–c) and soleus (e–g) muscle tissue sections of sham/no burn (a, e), burn/vehicle (b, f), and burn/HMBGB1 Ab (c, g) rats. Average histological score values of plantaris (d) and soleus (h) muscles were derived from analysis of n = 4 rats per group with two slides per tissue per rat, 36 microscopic fields per slide per experiment. Data in d & h are presented as dot plot and mean values ± SEM are shown with horizontal lines. Significance was calculated by one way ANOVA with Tukey’s post-hoc test and p-values are plotted as *p < 0.05 and **p < 0.01.

Back to article page