Figure 8
T cells expression of costimulatory molecules in burn rats (± HMGB1 Ab). Sprague Dawley rats were subjected to burn, immediately treated with one dose of vehicle or anti-HMGB1 antibody and euthanized at 3 days post-burn (controls: sham burn). Single cell suspensions of peripheral blood mononuclear cells (PBMC) and splenocytes were labeled with fluorescent-conjugated antibodies and analyzed by flow cytometry. Both αβ CD4+ T cells and γδ T cells were analyzed for the expression of CD80 and CD86 co-stimulation markers. Percentages of T lymphocytes that were αβ CD4+CD80+ (a, c), αβ CD4+CD86+ (b, d), γδ CD80+ (e, g) and γδ CD86+ (f, h) in peripheral blood (a, b, e, f) and spleen (c, d, g, h) of sham, burn, and burn/HMGB1 Ab-treated rats are shown. Representative images above the bar graphs show the mean fluorescence intensities of the target surface molecules in each group of rats. Data in bar graphs are derived from n ≥ 6 rats per group and plotted as mean values ± SEM. Significance was calculated by 1-way ANOVA/Tukey’s post-hoc test or Kruskal–Wallis H/Dunn’s post-hoc test and annotated as * (sham vs. burn or burn/treated) and ^ (burn vs. burn/treated). One, two, and three symbol characters were used to represent the p-values of ≤ 0.05, ≤ 0.01, and ≤ 0.001, respectively.
