Figure 2 | Scientific Reports

Figure 2

From: The tardigrade protein CAHS D interacts with, but does not retain, water in hydrated and desiccated systems

Figure 2

In vitro gelation of CAHS D and its variants is reflected in vivo by the appearance of fiber-like condensates upon osmotic stress. Maximum intensity projections, and inserts, of HEK cells stability expressing mVenus, mVenus:CAHS, mVenus:FL_Proline, or mVenus:2x Linker. Cells were either cultured and imaged under normal non-stressed conditions, or under osmotically stressed conditions (0.5 M Sorbitol for 4 h). The appearance of fiber-like condensation in mVenus:CAHS D and mVenus:2x Linker, but not mVenus:FL_Proline, under osmotic stress conditions mirrors the gelling properties (or lack thereof) of these proteins in vitro36. White arrows indicate fiber-like condensates observed in CAHS D and 2x Linker expressing cells upon osmotic shock. Blue = hoechst (DNA), red = SIR tubulin (microtubules), green = mVenus (monomeric mVenus or protein of interest). Scale bars = 10 µm.

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