Table 3 Calculation of analytical eco-scale for the developed method.

From: Quality by design paradigm for optimization of green stability indicating HPLC method for concomitant determination of fluorescein and benoxinate

Reagents/instruments

Penalty points

Reagents

 Phosphoric acid

2

 Isopropanol

4

Instruments

 HPLC

1

 Occupational hazards

0

 Waste

3

 Total penalty points

∑10

 Analytical eco-scale score

90

Back to article page