Table 4 Comparison of the proposed method and reported methods for determination of BNX HCl and FLR sodium.
Reported method[22] | Reported method[23] | Proposed method | |
|---|---|---|---|
Stationary phase | Inertsil ODS (250 mm × 4.6 mm, 5 μm) column | 4 mm × 30 cm column that contains packing L1 | Eclipse plus C18 (100 mm × 4.6 mm × 3.5 μm) column |
Mobile phase | Acetonitrile and 50 mM potassium dihydrogen phosphate buffer containing 0.01% triethylamine (pH adjusted to 5.0) in a ratio 40:60 v/v | Sodium 1-pentanesulfonate dissolved in glacial acetic acid (40%) and acetonitrile containing triethanolamine (60%) and pH is adjusted to 3.0 with phosphoric acid | Mixture of 27% (v/v) isopropanol and 73% (v/v) 20 mM potassium dihydrogen phosphate buffer (pH adjusted to 3.0) |
Run time | 8 min | 19 min | 4 min |
Detection wavelength | 220 nm | 254 nm | 220 nm |
Linearity range (µg/mL) | 4.0–80.0 for BNX 1.0–50.0 for FLR | NA | 2.5–60.0 for BNX 1.0–50.0 for FLR |
Analytical eco-scale score | 81 | 81 | 90 |
