Table 1 Interactions tested in S. cerevisiae two-hybrid assay.
Interactions | Interacting proteins | pGADT7/pGBKT7 | Self-activation | Interaction |
|---|---|---|---|---|
1 | Cys3 + Met3 | pRCP099/pRCP106 (YL018) | – | Yes |
2 | Cna1-ΔC + Met3 | pRCP094/pRCP106 (YL019) | – | Yes |
3 | Cnb1 + Met3 | pRCP096/pRCP106 | – | No |
4 | Gpp2 + Met3 | pRCP088/pRCP106 | – | No |
5 | Cys3 + Met3ΔLZ | pRCP099/pRCP128 (YL028) | – | No |
6 | Cys3 + Met3ΔLZ | pRCP099/pRCP132 (YL029) | – | No |
7 | Cna1-ΔC + Met3ΔLZ | pRCP094/pRCP128 (YL026) | – | No |
8 | Cna1-ΔC + Met3ΔLZ | pRCP094/pRCP132 (YL027) | – | No |
9 | Positive control ( +) | pGADT7-T/pGBKT-53 | – | Yes |
10 | Negative control (–) | pGADT7-T/pGBKT7-Lam | – | No |
