Figure 5
Gene expression and protein expression analysis of human EFEMP2. (A) EFEMP2 gene expression in cells obtained from the small airway epithelium (GSE64614). Data are presented as the mean ± SD (nonsmoker, n = 91; smoker, n = 37, two-tailed unpaired t test). (B) EFEMP2 gene expression in cells obtained from the bronchial epithelium (GSE994). Data are presented as the mean ± SD (never smoker, n = 23; current smoker, n = 34, two-tailed unpaired t test). (C) Representative human lung section image of IHC for EFEMP2. Control IgG using the same patient sample. A rabbit IgG antibody was used under the same w/v concentration and condition for EFEMP2. Scale bars, 100 μm. (D) Representative comparison of IHC images for high and low EFEMP2 expression in lung arteries and the alveolar area. Arrows indicate locations of human pulmonary EFEMP2 in the artery. Scale bars, 100 μm. (E) Measurement of EFEMP2 protein levels in lung section images based on ImageJ analysis. Data are presented as the median with interquartile range (n = 32 per group, two-tailed Mann–Whitney test). (F) Patient populations with high or low EFEMP2 levels based on IHC scoring of lung artery in the alveolar area (normal, n = 32; mild and moderate, n = 16, Chi-square/Fisher’s exact test with Benjamini–Hochberg false discovery rate procedure for multiple comparisons). ns, not significant. *P < 0.05, and **P < 0.05.
