Figure 3

SEM images of enamel discs showing: (A) 21 days growth of S. mutans (blue arrow) and A. viscosus (red arrow) without treatment. (B) biofilm growth (yellow circle) appeared after 21 days exposure to sterile saline for 10 min twice/day. (C) SEM image of the crystals (grey arrow) formed on the enamel surface after using probiotic-GSE nano emulsion-based Jelly candy and (D) was displayed covering of bacteria cells by Jelly candy (orange circle). In (E,F), it showed after magnified micrograph of (D) (orange circle) at magnification (× 4000) (E) presence of colony of cocci like bacteria (white circle). Magnification of (D) (orange circle) at (× 16,000) (F), it was clearly seen covering of S. mutans (dark orange arrows) by Jelly candy with the attachment of bacilli like bacteria (L. rhamnosus) (green arrows) on its surface.