Figure 5
From: Viability of HepG2 and MCF-7 cells is not correlated with mitochondrial bioenergetics
Effect of site-specific RC inhibition on the viability of HepG2 cancer cell line. (A) 24 h, 48 h and 72 h site-specific RC inhibition-induced cytotoxicity of HepG2 cells was assessed using nucleus-based quantification of dead (propidium iodide (PI) positive)/ total (Hoechst 33342 (Hoechst) positive) cells. (B) Effects of the pore-forming peptide alamethicin (Alam) and the detergent digitonin (Digit) on the viability of HepG2 cells. **p < 0.001 (one-way ANOVA, Dunnett’s test). (C) Mean number of HepG2 cells/field of view after 24 h, 48 h and 72 h site-specific RC inhibition. (D) Mean number of HepG2 cells/field of view after 15 min alamethicin or digitonin application. Bars indicate mean of at least three independent experiments, each addition with 12–32 statistical replicates and error bars indicate SEM. Concentrations of RC inhibitors were identical to those used in the metabolic profiling of HepG2 cells and see it in details in the legend of Fig. 3. *a: p < 0.05; *b: p < 0.001.
