Figure 6
From: Viability of HepG2 and MCF-7 cells is not correlated with mitochondrial bioenergetics
Effect of site-specific RC inhibition on the viability of MCF-7 cancer cell line. (A) 24 h, 48 h and 72 h site-specific RC inhibition-induced cytotoxicity of MCF-7 cells was assessed using nucleus-based quantification of dead (propidium iodide (PI) positive)/total (Hoechst 33342 (Hoechst) positive) cells. (B) Effects of the pore-forming peptide alamethicin (Alam) and the detergent digitonin (Digit) on the viability of MCF-7 cells. **p < 0.001 (one-way ANOVA compared to control, Dunnett’s test). (C) Mean number of MCF-7 cells/field of view after 24 h, 48 h and 72 h site-specific RC inhibition. (D) Mean number of MCF-7 cells/field of view after 15 min alamethicin or digitonin application. Bars indicate mean of at least three independent experiments, each addition with 12–32 statistical replicates and error bars indicate SEM. *p < 0.001 (one-way ANOVA compared to control, Dunnett’s test) Concentrations of RC inhibitors were identical to those used in the metabolic profiling of MCF-7 cells described in the legend of Fig. 3. For all other panels: *a: p < 0.05; *b: p < 0.001.
