Figure 8
From: Viability of HepG2 and MCF-7 cells is not correlated with mitochondrial bioenergetics
Effect of site-specific RC inhibition on apoptotic (and necrotic) markers in MCF-7 cancer cells. (A) 24 h, 48 h and 72 h site-specific RC inhibition-induced cytotoxicity of MCF-7 cells was assessed using nucleus-based quantification of dead (propidium iodide (PI) positive)/total (Hoechst 33342 (Hoechst) positive) and pSIVA positive (apoptotic) cells. Statistics are shown in Supplementary Table 2. (B) representative image of MCF-7 cells stained with Hoechst prior to any RC inhibitor treatment. (C) representative image of MCF-7 cells stained with Hoechst treated with SF 6847 for 72 h. (D) quantification of Hoechst area (pixels) of all images as the one shown in panel B of control MCF-7 cells. (E) quantification of Hoechst area (pixels) of all images as the one shown in panel C, treated with SF 6847 for 72 h. (F) quantification of all mean Hoechst areas obtained from all treatments for MCF-7 cell cultures. **p < 0.001 compared to control (one-way ANOVA, Dunnett’s test).
