Figure 3

Amplification profile and anneal derivative curve comparison of ACP gBlock gene fragment (synthetic positive control), and samples of D. citri. (a) Amplification profile of D. citri samples at 16 and 22 min and gBlock 1 × 106 copies/µL, at ~ 8 min (pink). Negative, no amplification (purple). (b) Anneal derivative of LAMP amplicons showing two peaks, ~ 78.5 °C for D. citri and ~ 83 °C for the gBlock (pink).