Figure 4

Optimised ACP 16S LAMP assay results using 1:8:4 primer ratio. (a) Positive amplification profile of 5 D. citri samples within 10 to 20 min and one non-target Trioza species no amplification (blue). Negative flat line (purple). (b) Anneal derivative at ~ 78.5 °C for 5 D. citri samples. Negative and Trioza species, no anneal peak (purple and blue).