Table 1 Asian citrus psyllid (ACP) 16S LAMP primer and amplicon (gBlock) sequences and parameters.

From: LAMP assay for the detection of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psylloidea: Psyllidae)

Primer

Sequence 5′–3′

Primer Length (bp)

Predicted Tm, annealing temperature °C

Degeneracy of primer (fold)

ACP_16S_F3

TATGTCCTGCTCAATGCTG

19

50

None

ACP_16S_B3

AATATTATGCTGTTATCCCTAAGGTA

26

59

None

ACP_16S_Floop

CATACCAGCCCCCAATTAA

54

73.5

None

ACP_16S_Bloop

GGTTGGGGTGACATAAAAT

43

77.2

None

ACP_16S_FIP

AATAAAAAAGTTAATATTACGTTCATCCACAAAGGTAGCATAATCATTAGTTCT

19

62.1

None

ACP_16S_BIP

GACGAGAAGACCCTATAGAATTTTTCAGGATCATCCAATCATC

19

58.6

None

gBlock Fragment

cccTATGTCCTGCTCAATGCTGcccACAAAGGTAGCATAATCATTAGTTCTcccTTAATTGGGGGCTGGTATGcccGGATGAACGTAATATTAACTTTTTTATTcccGACGAGAAGACCCTATAGAATTTcccGGTTGGGGTGACATAAAATcccGATGATTGGATGATCCTGAAcccTACCTTAGGGATAACAGCATAATATTccc

207

N/A

N/A

  1. The F2 and B2 primer regions of the FIP and BIP primers are marked in bold and underlined. Lowercase letters in the gBlock indicate extra c’s added between LAMP primer sites to increase the overall Tm of the amplicon.
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