Table 2 Panel of psyllid specimens used for developing the ACP 16S LAMP assay.

From: LAMP assay for the detection of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psylloidea: Psyllidae)

Species

Family

ID

COI Acc. #

ACP 16S LAMP

Time (min)

Temp (oC)

+/−

Diaphorina citri *

Psyllidae

1/1 F Thailand

OP185137

13.5

78.7

 + 

Diaphorina citri *

Psyllidae

2/1 Pakistan

OP185138

12.75

78.3

 + 

Diaphorina citri *

Psyllidae

2/2 F Pakistan

OP185139

11

78.4

 + 

Diaphorina citri *

Psyllidae

3/1M USA

OP185140

14.5

78.3

 + 

Diaphorina citri *

Psyllidae

3/3 USA

OP185141

10.5

78.3

 + 

Diaphorina citri *

Psyllidae

3/4 USA

OP185142

10

78.7

 + 

Agelaeopsylla sp. A

Aphalaridae

VAITC 9996

ON970392

  

Agelaeopsylla sp. B **

Aphalaridae

VAITC 10330

OP804507

  

Anoeconeossa sp. A

Aphalaridae

VAITC 10003

ON970387

  

Anoeconeossa sp. B

Aphalaridae

VAITC 8066

ON970385

  

Aphalaridae sp. A

Aphalaridae

VAITC 8082b

ON970381

  

Aphalaridae sp. B

Aphalaridae

VAITC 10002

ON970386

  

Aphalaridae sp. C

Aphalaridae

VAITC 8063

ON970390

  

Aphalaridae sp. D

Aphalaridae

VAITC 8030

ON970394

  

Blastopsylla sp.

Aphalaridae

VAITC 8098

ON970391

  

Cardiaspina bilobata

Aphalaridae

VAITC 10,001

ON970393

  

Cardiaspina fiscella

Aphalaridae

VAITC 8043b

ON970399

  

Cardiaspina fiscella **

Aphalaridae

VAITC 10332

OP804506

  

Cardiaspina retator

Aphalaridae

VAITC 8049b

ON970398

  

Cryptoneossa sp.

Aphalaridae

VAITC 8191

ON970382

  

Ctenarytaina sp. A

Aphalaridae

VAITC 8047b

ON970402

  

Ctenarytaina sp. B

Aphalaridae

VAITC 8194

ON970400

  

Glycaspis brimblecombei

Aphalaridae

VAITC 8088

ON970396

  

Glycaspis sp. A

Aphalaridae

VAITC 8232

ON970389

  

Glycaspis sp. B

Aphalaridae

VAITC 10000

ON970388

  

Glycaspis sp. C

Aphalaridae

VAITC 7587

ON970397

  

Glycaspis sp. D

Aphalaridae

VAITC 8093b

ON970395

  

Phellopsylla sp. A

Aphalaridae

VAITC 7554

ON970405

  

Phellopsylla sp. B

Aphalaridae

VAITC 9155

ON970401

  

Phellopsylla sp. C

Aphalaridae

VAITC 8058

ON970404

  

Spondyliaspis sp. A

Aphalaridae

VAITC 10004

ON970383

  

Spondyliaspis sp. B

Aphalaridae

VAITC 9999

ON970384

  

Mycopsylla fici**

Carsidaridae

VAITC 10331

OP804505

  

Acizzia conspicua

Psyllidae

VAITC 7222d

ON970415

  

Acizzia hakeae

Psyllidae

VAITC 7893a

ON970413

  

Acizzia sp. A

Psyllidae

VAITC 7900a

ON970412

  

Acizzia sp. B

Psyllidae

VAITC 7886

ON970414

   

Cacopsylla sp.

Psyllidae

VAITC 7511

ON970418

  

Aacanthocnema dobsoni

Triozidae

VAITC 9994

ON970410

  

Bactericera cockerelli *

Triozidae

VAITC 8404a

ON970403

  

Casuarinicola sp.

Triozidae

VAITC 8407

ON970411

  

Pauropsylla sp. A

Triozidae

VAITC 8406

ON970409

  

Trioza adventicia

Triozidae

VAITC 9933

ON970406

  

Trioza erytreae *

Triozidae

S. Africa

OP185136

  

Trioza melaleucae

Triozidae

VAITC 9992

ON970408

  

Trioza sp. A

Triozidae

VAITC 8057

ON970407

  

Triozidae sp. A

Triozidae

VAITC 9995

ON970416

  

Triozidae sp. B

Triozidae

VAITC 7589

ON970417

  

  1. For each specimen, species and family are reported, as well as the identification, the accession number for the COI sequences generated in this study and the results of the ACP 16S LAMP assay. The COI sequences presented here are the same as used in Fig. 2, and have been used to provide DNA barcoding identification of psyllid species. Bold indicates the target species, Diaphorina citri.
  2. *Exotic species.
  3. **This species was tested in the field using the QuickExtract DNA extraction method.