Figure 3 | Scientific Reports

Figure 3

From: Characteristic impairment of progesterone response in cultured cervical fibroblasts obtained from patients with refractory cervical insufficiency

Figure 3

Comparison of PR expression in the control and CI groups. (A) Quantitative RT-PCR using UCFs of the control and CI groups incubated in the presence of beta-oestradiol (10 nmol//L) for 24 h. Data are expressed as the mean ± SEM and analysed using the ratio paired t-test. *Indicates p < 0.05. (B) Western blotting using 10 μg of protein extracted from cells cultured without or with beta-oestradiol (10 nmol//L) PR-A:90 kDa, PR-B:118 kDa. Images of PR and GAPDH staining were obtained using the same lane of the same gel, with an exposure time of 300 s for PR and 60 s for GAPDH. (C) Immunocytochemistry images of UCFs cultured for 24 h with beta-oestradiol (10 nmol/L) using the anti-PR-A/B and anti-DAPI antibodies for nuclear staining. Scale bars: 10 μm.

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