Figure 3

(A) Size distribution of the projection maps approximated as elliptic circles illustrated in the top right panel. Open and filled circles indicate the size of the maps from the first (exp1) and second experiments (exp2). In addition, the symbols are colored with respect to the internal fine structures type-α (red), type-β (green), type-γ (blue) and type-δ (purple) shown in panel (C). In the frequency distributions of the long and short axes, the gray and white bars indicate the data from the first and second experiment, respectively. The dashed lines define the borders regarding the ARs of elliptic circles approximating the map shapes as circular shapes ‘C’ (1 < AR < 1.2), semi-circular shapes ‘S’ (1.2 < AR < 1.4), and elliptic shapes ‘E’ (1.4 < AR). The number of maps composing each category is shown in parenthesis. Here after, the labels at the upper left corner of each map indicates the first/second experiment (exp1/expp2), and the scale bar in each map indicates 200 nm. (B) Electron density profiles in a representative map (upper panel). The blue curves show the profiles along the dashed lines. The red curves are the profiles of projection density of ellipsoids with uniform density52. Peaks with approximate width of 150, 100, and 50 nm are indicated by black, green, and magenta bars, respectively. (C) Representative projection electron density of the types-α, β, γ, and δ maps. In each map, the label at the top right corner indicates the shape of the map classified according to the AR in panel (A). Characteristic fine-structures of major and minor high-density regions described in the main text are labeled in the magnified views of representative projection maps.