Table 1 Repeatability and Reproducibility Assays.

From: Development of quantitative multiplex RT-qPCR one step assay for detection of hepatitis delta virus

Standard (copies/reaction)

1st run (Ct)

2nd run (Ct)

3rd run (Ct)

Mean

SD ( ±)

CV(%)

1 × 105

19,722

19,811

19,853

19,80

0,07

0,34

1 × 104

22,973

23,106

23,128

23,07

0,08

0,36

1 × 103

26,244

26,552

26,382

26,39

0,15

0,58

1 × 102

29,691

29,788

29,872

29,78

0,09

0,30

1 × 101

33,207

33,388

33,431

33,34

0,12

0,36

5 × 100

34,257

34,330

34,324

34,30

0,04

0,12

Slope

− 3,321

− 3,401

− 3,373

–

–

–

Intercept

36,288

36,749

36,639

–

–

–

R2

0,999

0,999

0,999

–

–

–

Efficiency

100,04%

96,78%

97,89%

–

–

–

  1. The reproducibility and repeatability assays were performed in three runs on alternate days with dilutions in technical octuplicates, with coefficient of variation (CV%) less than 10% between runs.
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