Figure 1 | Scientific Reports

Figure 1

From: Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation

Figure 1

Representative serial histologic sections from dog Biceps femoris muscle. Transverse sections from healthy (Golden Retriever; GR), dystrophic (Golden Retriever Muscular Dystrophy; GRMD) and MuStem cell-treated dystrophic GRMD (GRMDT) dogs were stained with hematoxylin–eosin-saffron (HES; first row, arrowheads indicate mineral deposits in GRMD dogs) and immunolabeled for the developmental isoform of myosin heavy chain (MyHCd; second row, arrows indicate groups of positive fibers in GRMDT dog muscle). Connective tissue was visualized by specific Picrosirius red staining (third row). Sections were histoenzymologically stained to visualize nicotinamide adenine dinucleotide dehydrogenase-tetrazolium reductase (NADH-TR; bottom row) activity in mitochondria. The same labels (arrow, arrowhead) have been used on the four histological preparations (upper and lower panels) to highlight the features of interest that are preserved after serial sectioning of the tissues. Scale bars: 100 µm.

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