Figure 3 | Scientific Reports

Figure 3

From: Novel aminopyridazine derivative of minaprine modified by radiolysis presents potent anti-inflammatory effects in LPS-stimulated RAW 264.7 and DH82 macrophage cells

Figure 3

Effects of the aminopyridazine product on anti-inflammatory activities in LPS-stimulated RAW 264.7 macrophage cells. (A) Cell viability detection by MTT assay in RAW 264.7 cells after treatment with samples for 24 h. The cells were treated with samples for 2 h, followed by treatment with the LPS (0.1 μg/mL) for 24 h. (B) The nitrite content of culture media was analyzed by a Griess reagent assay. (C) The PGE2 production content of culture media was analyzed by ELISA. (D, E, F) Expression of COX-2 (E) and iNOS (F) induced by samples treatment of both RAW 264.7 macrophage cells for 24 h. (G, H, I) Cytokine productions (G: TNF-α, H: IL-6, I: IL-10) of culture media was measured by ELISA. (J, K) The iNOS (J) and Arg-1 (K) mRNA expression was determined by qPCR analysis. The relative expression of each gene was quantified using the expression level of GAPDH mRNA. The results are expressed as mean ± SD (n = 3). *p < 0.05; **p < 0.01; vs. LPS-treated group. 1: minaprine, 2: minaprinol.

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