Figure 4 | Scientific Reports

Figure 4

From: Novel aminopyridazine derivative of minaprine modified by radiolysis presents potent anti-inflammatory effects in LPS-stimulated RAW 264.7 and DH82 macrophage cells

Figure 4

Effects of the aminopyridazine product on anti-inflammatory activities in LPS-stimulated DH82 macrophage cells. (A) Cell viability detection by MTT assay in DH82 cells after treatment with samples for 24 h. The cells were treated with samples for 2 h, followed by treatment with the LPS (1 μg/mL) for 24 h. (B) The nitrite content of culture media was analyzed by a Griess reagent assay. (C) The PGE2 production content of culture media was analyzed by ELISA. (D, E, F) Expression of COX-2 (E) and iNOS (F) induced by samples treatment of both DH82 macrophage cells for 24 h. (G, H, I) Cytokine productions (G: TNF-α, H: IL-6, I: IL-10) of culture media was measured by ELISA. The results are expressed as mean ± SD (n = 3). *p < 0.05; **p < 0.01; vs. LPS-treated group. 1: minaprine, 2: minaprinol.

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