Figure 2 | Scientific Reports

Figure 2

From: Comparison of commercially available differentiation media on cell morphology, function, and anti-viral responses in conditionally reprogrammed human bronchial epithelial cells

Figure 2

Ion transport measurements in AB-ALI and PN-ALI differentiated CRpBECs. (A) Representative Ussing chamber short circuit current (Isc) tracings from the same donor recorded at 37 °C for AB-ALI (blue line) and PN-ALI (dashed blue line) cultures. Mean values of (B) baseline short circuit currents (ΔIsc), (C) Amiloride inhibited ENaC currents, (D) Forskolin/IBMX activated CFTR, (E) CFTRinh172 inhibited CFTR and (F) ATP-activated CaCC currents. n = 5 for AB-ALI and n = 4 for PN-ALI. Data presented as mean ± SEM. A color-matched symbol in both groups represents an individual donor. Data were analyzed using a Wilcoxon matched-pairs signed rank test, p < 0.05 was considered significant.

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