Figure 4 | Scientific Reports

Figure 4

From: A novel method of rapid detection for heavy metal copper ion via a specific copper chelator bathocuproinedisulfonic acid disodium salt

Figure 4The alternative text for this image may have been generated using AI.

Preparations of standard paper strips and standard curve. Standard solutions of BCS-Cu+ were prepared under optimized reaction conditions (50 mM Tris–HCl pH 7.4, 200 µM BCS, 1 mM ascorbate) with varying concentrations of CuSO4 (0, 0.2, 0.5, 1, 2, 5, 10, 20 mg/L). (A) Standard solutions were imaged in glass test tubes. (B) A standard curve of BCS-Cu+ was prepared by measuring the absorbance at 490 nm over concentration. (C) Paper strips (filter paper, 0.5 × 6 cm) were soaked in standard solutions of BCS-Cu+, dried for 1 min and visualized against a white background.

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