Figure 4 | Scientific Reports

Figure 4

From: A novel method of rapid detection for heavy metal copper ion via a specific copper chelator bathocuproinedisulfonic acid disodium salt

Figure 4

Preparations of standard paper strips and standard curve. Standard solutions of BCS-Cu+ were prepared under optimized reaction conditions (50 mM Tris–HCl pH 7.4, 200 µM BCS, 1 mM ascorbate) with varying concentrations of CuSO4 (0, 0.2, 0.5, 1, 2, 5, 10, 20 mg/L). (A) Standard solutions were imaged in glass test tubes. (B) A standard curve of BCS-Cu+ was prepared by measuring the absorbance at 490 nm over concentration. (C) Paper strips (filter paper, 0.5 × 6 cm) were soaked in standard solutions of BCS-Cu+, dried for 1 min and visualized against a white background.

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