Figure 5

Differences in the cecal microbial composition and gene expression of broiler ilea between the two groups. (A) Bacterial community compositions at phylum level. (B) Bacterial community compositions at genus level. (C) Manhattan plots show cecum-enriched ASVs in Group C. All dots and circles in the figure occur at frequencies ≥ 0.3 in two groups; ASVs above the dashed line are significantly different between two groups. The solid dots represent ASVs that are significantly upregulated in Group C, while hollow circles represent ASVs that are significantly downregulated in Group C. The ASVs that are not significantly different between two groups are indicated below the dashed line (gray circles). The size of dot/circle represents abundance; different ASVs are colored by phylum level. (D) The gene expression of broiler ileal absorption. The results are expressed as the means ± SDs, n = 3. *Indicates a difference at p < 0.05, **at p < 0.01, ***at p < 0.001.